Host: Anacystis nidulans and Synechococcus cedrorum
May be causative agent of disappearance of cyanobacteria (biocontrol)
Isolated from wastewater stabilization pond in Florida
Structure: resembles T even bacteriophage, icosahedral protein coat, long contractile tail and 91 kb double
stranded DNA with GC content of 53-54%
The DNA metabolism of AS-1 with its phage-induced light (PIL) DNA has previously been studied
Current interest: molecular cloning of AS-1 DNA and phylogenetic comparison with other marine and fresh water
cyanophage and cyanobacteria
One of the goals of this project is to use specific sequences of AS-1 DNA as a probe to detect the presence
of AS-1 in aquatic environments